波长型SPR检测仪的灵敏度探讨

Studies on the Sensitivity of a Wavelength-dependent SPR Sensor

利用自行设计构建的可变入射角的波长型表面等离子体共振(SPR)检测仪,在不同浓度的蔗糖溶液中测定了不同入射角度(80°～66°)的共振曲线,经过处理得到共振峰位、半高宽及灵敏度随入射角和样品折射率变化的三维图像.在此基础上探讨了波长型SPR检测仪的主要参数对仪器性能的影响,从理论和实验上证明了影响灵敏度的主要因素为共振波长,并且随着共振波长的增大,检测灵敏度迅速提高.

A wavelength-dependent surface plasmon resonance（SPR） sensor with tunable incident angles was designed and constructed.The resonance curves of sucrose solutions at different concentrations were measured under different incident angles（80°—66°）.Several 3-dimensional（3D） maps were obtained through the experiment data,which reflects the evolution of the resonance wavelength,full width at half maximum（FWHM） and sensitivity as functions of refractive index and incident angle.The quality of the wavelength-dependent SPR sensor was systematically discussed via the theoretical simulation and experimental data.It indicates that the sensitivity increases rapidly with the red-shift of resonance wavelength.Through these 3D maps,the resonant wavelength,FWHM and sensitivity of analyses can be predicted.These 3D maps could give suggestions to the design and construction of wavelength-dependent SPR devices.