盐酸吉西他滨对乳腺癌小鼠Tusc2、Lats2基因表达的影响

Gemcitabine (Gemzer) up-regulates the expression of gene Tusc2 and Lats2 in breast cancer mice

目的探讨盐酸吉西他滨对乳腺癌小鼠肿瘤抑制因子候选基因2(Tusc2)、大型肿瘤抑制因子2(Lats2)基因mRNA及蛋白水平表达的影响。方法首先建立小鼠乳腺癌模型,盐酸吉西他滨药物处理前(0d)、处理后第5天(5d)、处理后第10天(10d)分别取材,采用RT-PCR和Western blotting方法检测Tusc2、Lats2基因表达量的变化。结果盐酸吉西他滨作用乳腺癌小鼠第10天(10d)后,RT-PCR结果显示,与空白对照组相比,实验组Tusc2与Lats2 mRNA相对表达量分别增加约4.5倍与8倍(P<0.05),其余各组与相应空白对照组相比,相对表达量无统计学差异(P>0.05);同时Western blotting结果显示,实验组与空白对照组相比,TUSC2与LATS2蛋白相对表达量分别上调约10倍与5倍(P<0.05)。其他各组之间相比统计学无显著差异(P>0.05)。结论盐酸吉西他滨能有效上调乳腺癌小鼠体内Tusc2、Lats2基因的表达。

Objective To explore gemcitabine＇ s effect on tumor suppressor candidate 2 （Tusc2）, large tumor suppressor2 （Lats2） gene expression in breast cancer of mice, both in mRNA and protein level. Methods Mice models of breast cancer were established and the samples were selected at 0 day, 5 days and 10 days after gemcitabine hydrochloride treatments as well as control. RT-PCR and Western blotting were used to detect the variation of the Tusc2 and Lats2 gene expression both for blank control, negative control and test groups. Results The results were similar both in RT-PCR and Western blotting. Compared with blank control, relative amounts of Tusc2 and Lats2 RNA in test group increased around 4.5 and 8 times 10 days after treated with gemcitabine in breast cancer mice respectively （ P 〈 0.05 ）. However, no differences were showed with Tusc2 and Lats2 RNA level 5 days after treated with gemcitabine in breast cancer mice. As to protein expression level, TUSC2 and LATS2 protein relative amounts were raised around 5 and 10 times （P 〈 0. 05） compared with blank control. Significant statistical difference was found between test group and control （P 〉 0. 05 ）. No significant differenc was found between blank control and negative control （P 〉 0. 05 ）. Conclusion Gemcitabine hydrochloride can upgrade Tusc2 and Lats2 gene expression effectively in breast cancer mice.