小鼠自然毛囊生长周期及β连环蛋白的表达

Hair follicle growth cycle and the expression of β-catenin in mice

目的观察昆明系小鼠自然毛囊生长周期的组织学特点,探讨β连环蛋白(β-catenin)在小鼠自然毛囊生长周期中的表达规律及其与毛囊生长更替的关系。方法采用石蜡切片HE染色、Western blotting以及免疫组织化学方法,研究小鼠(90只)毛囊的周期性变化,检测β-catenin在小鼠毛囊生长不同时期皮肤组织中的蛋白表达水平,并对其进行组织定位。结果处于不同生长时期的毛囊具有各期的特殊结构;Western blotting结果显示,在小鼠皮肤组织粗蛋白提取物中存在分子量约为85kD且与兔抗β-catenin多克隆抗体发生免疫阳性反应的蛋白条带;免疫组织化学结果显示,β-catenin在不同毛囊生长时期的表皮、皮脂腺、根鞘和真皮乳头均有不同程度的表达。结论昆明系小鼠毛囊生长呈周期性变化,β-catenin在毛囊生长周期中的阳性反应与毛囊的周期性变化存在相关性,表明β-catenin在昆明系小鼠自然毛囊生长周期中起维持、促进毛囊生长的作用。

Objective To observe the histological characteristics of murine hair follicle in distinct hair follicle cycle stage, and to research the function of β-catenin in murine hair follicle cycle by investigating the expression and localization of β-catenin in murine distinct hair follicle cycle stage. Methods HE staining was used to observe the morphology of the murine hair follicle in each stage （ n = 90） , Western blotting and immunohistochemistry were used to determine the expression level and localization of β-catenin protein in distinct hair follicle cycle stage. Results For each stage, stage-specific characteristics were provided in order to classify murine hair follicle cycle. The distribution of β- catenin in murine skin was demonstrated. The positive reaction of β-catenin was detected in epidermis, sebaceous glands, root sheath and dermal papilla. Conclusion The growth of murine hair follicle shows cyclical variation. The expression of β-catenin is significantly different in distinct hair follicle cycle stage suggesting that telogen-to-anagen transition depends on the expression level of β-catenin. β-catenin is one of the moleculars that maintain anagen and promote telogen-to-anagen transition.