ECM-1和VEGF在胃癌中的表达

Expression of extracellular matrix protein 1 and vascular endothelial growth factor in gastric cancer

目的探讨细胞外基质蛋白1(ECM-1)和血管内皮生长因子(VEGF)在胃癌中的表达及其临床意义。方法分别采用免疫组化法和Western blot法检测60例手术切除的胃癌组织及癌旁正常组织中ECM-1和VEGF的表达水平。结果与癌旁正常组织相比,ECM-1和VEGF在胃癌组织中的阳性表达率分别为86.7%和78.3%,差异有统计学意义(P<0.05)。ECM-1的阳性表达与肿块大小、浸润深度、淋巴结转移和TNM分期显著相关(P<0.05),与患者的性别、年龄和肿瘤的分化程度差异无统计学意义;VEGF的阳性表达与肿瘤的浸润深度、淋巴结转移和TNM分期显著相关(P<0.05),与患者的性别、年龄、肿块大小和分化程度差异无统计学意义(P>0.05)。两指标在胃癌组织中的表达水平呈正相关(rs=0.753,P<0.05)。结论 ECM-1和VEGF在胃癌组织中呈正相关表达,与其侵袭转移相关,ECM-1可能通过上调VEGF的表达促进胃癌的肿瘤血管生成。

Objective To explore the relationship between the expression of extracellular matrix protein 1 （ECM- 1 ） and vascular endothelial growth factor （VEGF） and clinicopathological features in gastric cancer. Methods The expression of ECM-1 and VEGF proteins in 60 gastric carcinomas and tumor-adjacent normal tissues were detected by immunohistochemical staining and Western blot methods. Results Compared with the tumor-adjacent normal tissues, the positive expression rate of ECM-1 and VEGF was 86.7% and 78.3% , respectively. And there was significant difference between two groups （ P 〈 0. 05 ）. The expression of ECM-1 was closely correlated with tumor size, depth of invasion, metastasis of lymph nodes and TNM stage （P 〈 0. 05）, but no association was observed with gender, age, size,pathological grades. The expression of VEGF was related to the depth of invasion, metastasis of lymph nodes and TNM stage （ P 〈 0.05 ）, but not related to the gender, age, pathological grades and tumor size （P 〉 0. 05 ）. There was significant positive correlation between the expressions of two indicators in gastric cancer （r = 0. 753, P 〈 0. 05 ）. Conclusion The expression level of ECM-1 and VEGF may correlate with the invasion and metastasis of gastric cancer, which may be associated with the up-regulation of VEGF induced by ECM-1.