外感湿热证患者Th1/Th2细胞因子水平变化的研究

Study on Th1/Th2 Level of Patients with Exogenous Damp-heat Syndrome

目的:通过观察外感湿热证患者外周血T细胞免疫水平的变化,探讨外感湿热证的部分发病机制。方法:30例均为外感发热患者,辨证属外感湿热证者。正常对照组15例,为同时期健康志愿者。采用酶联免疫吸附试验(ELISA)检测患者与健康者血清白介素4(IL-4)、干扰素γ(IFN-γ)水平,运用蒿芩清胆汤治疗患者3天,复测上述指标,观察蒿芩清胆汤的干预作用。结果:经蒿芩清胆汤治疗后,外感湿热组血清IFN-γ水平显著降低(P<0.01),INF-γ/IL-4比值明显降低(P<0.01)。结论:提示Th1/Th2比值失衡可能是外感湿热证发病机制之一,调整Th1/Th2失衡是蒿芩清胆汤治疗外感湿热证的机制之一。

Objective. To explore the pathogenic mechanism of exogenous damp-heat syndrome by studying T lymphocyte subtypes of Th1/Th2 in peripheral blood. Methods： Thirty externally contracted.fever patients with exogenous damp-heat syndrome, serving as exogenous damp-heat （EDH） group, were treated with Hao Qin Qingdan Decoction for three days. Fifteen healthy volunteers served as the control group. The serum levels of interleukin-4 （IL-4） and interferon gamma （IFN- γ） in patients and healthy volunteers were detected by enzyme linked immunosorbent assay （ELISA）. Results： After treatment, IL-4 level and INF-γ/IL-4 ratio were markedly decreased in EDH group （P 〈 0.01 compared with those before treatment）. Conclusion= The disequilibrium of Th1/Th2 ratio is probably one of the pathogenic mechanisms of fever due to exogenous damp-heat. To adjust the disequilibrium of Th1/Th2 ratio is one of the possible therapeutic mechanisms of Hao Qin Qingdan Decoction in treating fever due to exogenous damp-heat.