牛血浆FN对人牙髓成纤维细胞骨形成蛋白和碱性磷酸酶分泌和表达的影响

Effects of Bovine Plasma Fibronectin on Expression of Bone Morphogenetic Protein and Alkaline Phosphatase in Cultured Human Dental Pulp Cells

目的：观察纤维粘连蛋白（ＦＮ）与牙髓细胞分化的关系．方法：将传代的人牙髓细胞接种于９６孔板，分别用不同浓度牛血浆ＦＮ（１０，２０，４０，８０μｇ／ｍｌ）作用３天，进行骨形成蛋白（ＢＭＰ）和碱性磷酸酶（ＡＬＰ）的免疫组化染色，图象分析仪半定量测定，并测定培养上清液中ＡＬＰ活性．结果表明：四种浓度的ＦＮ组的牙髓细胞ＢＭＰ和ＡＬＰ表达无明显差异，ＦＮ（２０，４０μｇ／ｍｌ）组培养上清液中ＡＬＰ活性较强．结论：ＦＮ在牙髓细胞分化过程中的作用是有限的．

Aim: To observe the rclationship between fibronectin(FN)and the differentiation of the dental pulp cells.Methods:The human pulp cells at passages 5 were used for the experiments,the cells at the proliferating stage were seeded on the plastic 96-well plates.Four concentrations of FN(10,20,40,80g/ml)were added to the fresh DMEM supplemented with 1%FCS,then the cultivation were continued for 3d,the cells were fixed and then ABC-peroxidase labeling were used to study the intracellular production of alkaline phosphatase(ALP)and bone morphogenetic protein(BMP),ALP activity of culture supernatnant were also measured.Result:There were no signifcant difference on expression of BMP,ALP in FN groups,but FN(20,40g/ml)stimulated ALP activity in supernatant.Conclusion:The role of FN involved in differentiation of dental pulp cells may be limited.