α-硫辛酸对大鼠视网膜缺血再灌注损伤中P53和Bax表达的影响

Effect of α-Lipoic acid on Bax and P53 in rat retina after ischemia-reperfusion injury

目的:观察α-硫辛酸在大鼠视网膜缺血再灌注损伤(retinal ischemia-reperfusion injury,RIRI)过程中对P53和Bax表达的影响。方法:将SD大鼠72只随机分成正常组、视网膜缺血再灌注组和α-硫辛酸干预组。同时后两组根据再灌注的时间不同分成6,24,48和72h组。通过前房灌注加压的方法制成RIRI动物模型,采用Western-blot与免疫组织化学法测定在大鼠RIRI模型中的P53和Bax表达的变化。结果:P53和Bax在正常视网膜组织中几乎不表达,在缺血灌注6h开始表达,24h达到高峰,48h开始下降,72h后表达已经明显减弱。α-硫辛酸干预组各观察指标变化趋势基本与缺血再灌注组相似,但表达明显减弱,两组间比较差异均有显著统计学意义(P<0.01)。结论:α-硫辛酸可抑制P53和Bax的表达,对大鼠RIRI有保护作用。

AIM：To investigate the effect of α-lipoic acid（α-LA） on the retinal morphology expression of P53 and Bax during retinal ischemia-reperfusion injury. METHODS：A total of 72 SD rats were randomly divided into normal control group, ischemia- reperfusion model group and LA treatment group. the last two groups were subdivided in group 6 hours,24 hours,48 hours,72 hours after reperfusion. The models of retinal ischemia-reperfusion injury were made by elevating intraocular pressure. Immunohistochemistry and Western-blot were used to measure changes of P53 and Bax protein levels in retinal tissue. RESULTS： No expression of P53 and Bax positive cells were found in normal group, at the ischemia-reperfusion groups.the expression of P53 and Bax began to increase at 6 hours after reperfusion, at 24 hours reached the peak, began to decrease at 48 hours,and weakened obviously at 72 hours . The therapy groups of α-lipoic acid have the same trend with the ischemic groups in each index, but the expressive intensity of each period weakened in evidence. There was significant difference （P〈0.01）. CONCLUSION： P53 and Bax play an important role in the happening of retinal ischemia-reperfusion injury （RIRI）;α-LA can inhibit the expression of P53 and Bax cell apoptosis.