滇桂艾纳香水溶性多糖BRP-B的分离纯化及止血活性

Isolation,Purification and Hemostatic Activity of Water-soluble Polysaccharide in Blumea Riparia(BI.) DC.

用蒸馏水溶解滇桂艾纳香浸膏,从中提取多糖,通过膜分离、三氯乙酸法脱蛋白、二乙氨基乙基(DEAE)纤维素脱色、SephadexG10、SephadexG50凝胶柱色谱纯化,获得滇桂艾纳香水溶性多糖(BRP-B)。由凝胶渗透色谱(HPGPC)确定BRP-B为相对分子质量分布均一的多糖,其数均分子量和质均分子量分别为2654和2716Da。以血浆复钙时间(PRT)为指标,研究BRP-B的凝血活性,结果显示BRP-B质量浓度为6.25×10-2g/L时,凝血时间为248.52s,凝血时间抑制率达22.42%。小白鼠离体子宫实验结果显示,BRP-B质量浓度为1.67×10-2、1.97×10-2及2.85×10-2g/L时能明显增加子宫平滑肌的收缩频率。

Water-soluble polysaccharide B（BRP-B） in Blumea riparia（BI.） DC.was extracted and purified by using membrane separation,discoloration on diethylaminoethyl（DEAE）-cellulose,and sephadex column chromatography of G10 and G50.Results from high-performance gel permeation chromatography（HPGPC） showed that the obtained BRP-B has a narrow-distributed molecular mass,with the number average molecular mass and mass average molecular mass as 2654 and 2716,respectively.The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6.25 × 10-2 g/L had the strongest procoagulant activity,the coagulation time was 248.52 s,and the inhibition rate of coagulation time was 22.42%.In particularly,BRP-B with concentration of 1.67 × 10-2,1.97 × 10-2 and 2.85 × 10-2 g/L could significantly enhance the frequency of uterus muscle.