汉族寻常型痤疮患者TNF-α-308 G/A基因多态性检测与分析

Detect and Analyze the TNF-α-308G/A Polymorphism of Acne Vulgaris in Han Chinese

目的探讨前炎症介质TNF-α-308G/A基因多态性与汉族寻常型痤疮患者的关联性,为汉族寻常型疮痤发病遗传学研究奠定基础。方法通过PCR-RFLP技术研究了138例寻常型痤疮患者和120例正常对照TNF-α-308 G/A基因型的分布以及等位基因的频率。结果发现寻常型痤疮组基因型GG(62.40%),GA(30.40%),AA(7.20%)的频率分布与对照组(80.90%,18.30%,0.80%)比较,差异均有统计学意义(P<0.05);等位基因A(22.50%)频率分布,寻常型痤疮组与对照组比较,差异有统计学意义(P<0.05)。结论 TNF-α-308 A等位基因的存在,增大了寻常型痤疮的发生风险。

Objective To explore the correlation between the TNF-a -308 G/A gene polymorphism and acne vulgaris of ttan chinese, and lay the fundation for genetic studies of acne vulgaris of Han Chinese. Methods A total of 138 acne vulgaris patients and 120 healthy subjects from han Chinese ethnic group were enrolled in the study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique was adopted to analyze the single nucleotide polymorphisms （SNPs） of TNF-a-308 G/A gene, and to examine the association between acne vulgaris and the polymorphisms in TNF-a-308 G/A gene. The relationship between different genotypes and the susceptibility of acne vulgaris was analyzed. Results There were significant difference in the frequency of the genotype GG, GA, AA in the TNF-a-308 G/A genetic polymorphisms between acne vulgaris patients and healthy control （P 〈 0. 05） ; and there was significant difference in the allele （A） frequency between acne vulgaris patients and healthy control （P 〈 0.05 ）. Conclusion The high freqnency of A allele in the funditonal 308 G/A polymorphism of TNF-a is a high risk of acne vulgaris in Han Chinese.