中药水煎剂对马拉色菌分离株的体外抑菌实验

Susceptibility Testing of Genus Malassezia In vitro to The Chinese Medicinal Lotion

目的观察苦参、百部、土槿皮、大风子、白藓皮、蛇床子、黄柏、地肤子及其混合液体外抑制马拉色菌的效果。方法参照M27-A方案中的酵母菌微量稀释法进行药敏试验。结果六种马拉色菌标准菌株对本水煎剂的敏感性:糠秕马拉色菌的MIC最低(0.062 5g/mL),其次为厚皮马拉色菌(0.125g/mL),球形马拉色菌(0.5g/mL),钝形马拉色菌(0.5g/mL),斯洛菲马拉色菌(0.5g/mL),合轴马拉色菌(>0.5g/mL)。结论复方中各单味药间对多株马拉色菌有相互协同作用,对合轴马拉色菌有拮抗作用。

Objective To test the effect of sophorae flavescentis, radix stemona japonica, pseudolarix, Hydnocarpus anthelmintica Pierre,dictamnus dasycarpus turcz, cnidium monnieri,phellodendron chinense, Kochia scoparia Schrad and its intermixture on the inhibition of the growth of Malassezia. Methods The susceptibility testing of Malassezia in vitro was performed by M27-A microdilution method. Results The susceptibility of 6 standard strains of Malassezia species to the Chinese medicinal lotion were Malassezia furfur （ MIC ： 0. 062 5g/mL）, Malassezia pachydermatis （ MIC ：0. 125g/mL） ,Malassezia globosa （ MIC ：0.5g/mL）, Malassezia obtusa（ MIC ：0.5g/mL ） , Malassezia slooffiae （ MIC ： 0. 5g/ml, ） , Malassezia sympodiali （ MIC ： 〉 0.5g/mL）. Conclusion The single ingredient in the compound has a synergic function against most Mal- assezias but a antagonist against Malassezia sympodiali.