旋毛虫肌蚴可溶性抗原与日本血吸虫抗血清交叉反应的研究

Study on Cross-Reaction Between Soluble Antigen of T.spiralis Muscular Larva and Anti-Schistosoma Japonicum Sera

【目的】从分子水平研究旋毛虫诱导抗日本血吸虫保护性免疫。【方法】应用ＥＩＴＢ技术对旋毛虫肌蚴上清抗原与多种日本吸虫抗血清的交叉反应进行分析。【结果】旋毛虫肌蚴抗原与日本吸虫感染血清、抗雄虫家兔血清、抗尾蚴血清、抗成熟卵及未成熟卵血清、抗环卵血清、抗成虫表膜血清及抗ＧＳＴ血清存在广泛交叉反应带。【结论】旋毛虫抗血吸虫抗原具有复杂性，其与血吸虫不同发育阶段、不同组分免疫血清存在交叉反应。

To investigate the protective immunity against Schistosoma japonicum(Sj) induced by Trichinella spiralis (Ts) from molecular level. Cross-reactions between supernatant antigen of T.spiralis muscular larva and several anti-Schistosoma japonicum sera were analyzed by means of SDS-PAGE and enzyme-linked immunoelectrotransfer blot (EITB). The antigens of T.spiralis were recognized by Schistosoma japonicum infected rabbit sera(SjIRS),anti-Sj male worm rabbit sera (aSj),anti-Sj cercaria (aSjcer),anti-soluble egg antigen (aSEA),anti-soluble immature egg antigen (aSIEA),anti-circumoval sera (aCos),anti-Schistosoma japonicum adult worm surface membranous antigen (aSjAWSMA) and anti-glutathione S-tranferase(AGST),showing extensive cross-reacting bands.The SjIRS displayed many bands of 3192 KDa which were found in Ts infected rabbit sera (TsIRS),aSj displayed at least 11 bands,especially those with 92,87,66,58 KDa.The aSIEA was charaterized by the appearance of legible 63 KDa band and densely stained 54 KDa band,while the aSEA by the presence of 58,57 KDa bands.Both antisera exhibited the same bands of 92,87,54,47 KDa.The aSjcer revealed a few bands unclearly(55,54, 52,49,47,31 KDa etc.).Bands with MW of 55,54,47,44 and 42 KDa were seen in aCos,whereas a distinct band of 54 KDa appeared in aSjAWSMA. The antigen of Ts against Schistosoma japonicum is very complex.It manifects cross-reactions with immune sera obtained from different developing stages and components of Sj.