人脐静脉内皮细胞膜上钙和钾离子通道

CALCIUM ION CHANNEL AND POTASSIUM ION CHANNEL IN MEMBRANE OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

目的 :观察体外培养人脐静脉内皮细胞膜上钙离子 ( Ca2 +)通道和 Ca2 +激活的钾离子 ( Kca)通道。方法 :膜片钳单通道记录技术。结果 :体外培养人脐静脉内皮细胞膜上含有 Ca2 +通道和 Kca通道 ,但其通道分布概率和开放概率均不高。结论 :人脐静脉内皮细胞作为一种非兴奋性细胞 ,其细胞膜上含有的 Ca2 +通道和 Kca通道 ,对细胞浆内Ca2 +浓度的维持、Ca2 +补充来源和细胞膜电位的维持等均具有重要意义 ,为进一步研究人脐静脉内皮细胞膜上通道的生理。

Objective: To observe the calcium ion channel and potassium ion channel in the membrane of human umbilical vein endothelial cells (HUVEC) cultured in vitro. Methods: Patch clamp technique was used. Results: There existed the calcium ion channel and potassium ion channel in the membrane of HUVEC, but the probabilities of distribution and opening of these channels were not high. Conclusion: The ion channels in the membrane of HUVEC play an important role in the maintanance of the concentration of intracytosolic free calcium and potassium ion.