头半棘肌肌球蛋白腺苷三磷酸酶与琥珀酸脱氢酶染色比较

A COMPARATIVE STUDY ON MYOSIN ADENOSINE TRIPHOSPHATASE AND SUCCINATE DEHYDROGENASE FOR MUSCULUS SEMISPINALIS CAPITIS

目的 :研究大鼠、家兔和人头半棘肌的肌纤维型分布。方法 :应用肌球蛋白腺苷三磷酸酶 ( ATP酶 )和琥珀酸脱氢酶染色法 ,观察比较各型肌纤维的组织化学特征。结果 :( 1) ATP酶染色 ,大鼠、家兔、正常人颈后部肌肉肌纤维可分为 I型和 II型纤维 ,呈多边形或椭圆形 ,分别约占 60 %和 4 0 %。 ( 2 )琥珀酸脱氢酶染色 ,肌纤维呈蓝色 ,交错排列 ,可分为深染、中染和浅染三型 ,分别约占 2 5%、2 5%和 50 % ,种系间无显著性差异。ATP酶活性反应肌纤维的收缩特性 ,而琥珀酸脱氢酶活性反应肌纤维的代谢特征 。

Objective: To observe the distr ibution of fiber types of the musculus semispinalis capitis in rats, rabbits and humans. Methods: The histochemical characteristics of various types of fibers of the musculus semispinalis capitis were observed by both myosin adenosine triphosphatase (ATPase) and succinate dehydrogenase (SDH) staining. Results: (1) ATPase: In rats, rabbits and humans, the fibers of the musculus semispinalis capitis were multi sided or ellipsoid and were divided into type I and type II, which accounted for 60% and 40% respectively. (2) SDH: The fibers were blue and divided into dark, mediate and light types, which were interweaved and multi sided or ellipsoid. The proportion of dark, mediate and light fibers was about 25%, 25% and 50% in rat, rabbit, and humans respectively. Conclusions: The activity of ATPase and SDHase showed the contractive and metabolic features of the muscles fibers.