HPLC法测定恶吐平制剂各组份含量

Simultaneous determination of injection E Tuping four components by HPLC

用反相色谱法分析山莨菪碱、甲氧氯普胺、地塞米松及苯海拉明四种组份，在甲醇－水流动相体系中加入少许三乙胺使Ｃ１８固定相表面的化学活性改变，结合选择流动相的适宜ｐＨ值，达到了满意的色谱分离效果。ＵＶ检测，其线性范围分别为：１０～２４０ｍｇ／Ｌ、１０～２４０ｍｇ／Ｌ，１０～２００ｍｇ／Ｌ，以及１５～２００ｍｇ／Ｌ，最大Ｒｓ≤３９４。

Simultaneous separation and analysis of four components of E Tuping-anisodamine; metoclopramid; dexamethasone and diphenhydramine by HPLC were reported. The reversed-phase C18 column (150mm4.6mm) was applied and the mobile phase was methanol-water (47:53) containing with 0.12% triethylamine, adjusted to pH 4.5 with phosphoric acid (about 0.25%).The compounds were detected using the UV detector operated at 220 nm which allowed linear ranges of determination 10240 mg/L,10240 mg/L,10200 mg/L and 15200 mg/L,respectively (the greatest Rs3.94).This method is stable,simple and fast.