EB病毒潜伏膜蛋白抑制鼻咽癌细胞分化的定量分析

Quantitative Analysis of Nasopharyngeal Carcinoma CellsDifferentiation Inhibited by EB Virus LMP

为了探讨ＥＢ病毒潜伏膜蛋白（ＥＢＶ－ＬＭＰ）对鼻咽癌（ＮＰＣ）细胞体外分化及分化诱导剂二甲基亚砜（ＤＭＳＯ）敏感性的影响。以ＥＢＶ－ＬＭＰ基因转染人高分化鼻咽癌细胞株（ＣＮＥ１），并用ＤＭＳＯ诱导，采用免疫组化及图像分析法观察细胞分化及形态的变化。结果显示：ＤＭＳＯ体外诱导ＣＮＥ１细胞生长明显受到抑制，细胞变大，核浆比值明显变小（Ｐ＜０．０１），细胞角蛋白表达显著增高（Ｐ＜０．０１）；ＬＭＰ在体外可明显促进ＣＮＥ１细胞的生长，细胞变小，核浆比值明显变大（Ｐ＜０．０１），细胞角蛋白表达显著下降（Ｐ＜０．０５），有向低分化鳞癌发展倾向；ＬＭＰ基因转染细胞诱导后体外生长和角蛋白表达无显著改变（Ｐ＞０．０５）。结果表明，ＤＭＳＯ可在一定程度上诱导ＣＮＥ１细胞分化；ＬＭＰ对ＣＮＥ１细胞生长有明显的促进作用，可抑制细胞分化；ＬＭＰ可降低ＣＮＥ１细胞对终末分化信号的反应。

EBV-LMP gene was transfected into human welldifferentiative NPC cell line CNE1 and induced by dimethyl sulfoxide(DMSO).In vitro,image analysis and immunohistochemical staining was used for determing of keratin expression and morphometry of cells.The results showed that the growth of CNE1 cells was inhibited after induction by DMSO,cell size increased,with a low nuclear/cytoplasm ratio(P<0.01),the keratin expression increased(P<0.01).The LMP promoted the growth of NPC CNE1 cells,decreased cell size,with a high nuclear/cytoplasm ratio(P<0.01),lower keratin expression(P<0.05),there was a trend to low differentiative carcinoma,there were less changes on morphometry and keratin expression after induction(P>0.05).It is suggested that DMSO may induce differentiation of CNE1 cells and LMP may promote the CNE1 cell growth and inhibit its differentiation,decrease the response of cells to terminal differentiative signals.