食道鳞癌凋亡相关基因表达及原位凋亡的同步检测

Multiple Apoptosisrelated Genes Expression and in Situ Detection of Apoptosis in Human Esophageal Squamous Cell Carcinoma

用免疫组织化学染色法对食道鳞癌标本进行凋亡相关基因Ｐ５３、Ｆａｓ、Ｂｃｌ２和Ｂａｘ表达的检测，同时采用ＴＵＮＥＬ方法原位探测细胞凋亡。检测结果显示：①５２例食道鳞癌标本中，Ｐ５３、Ｆａｓ、Ｂｃｌ２、Ｂａｘ和细胞凋亡的阳性率分别是６１．５％、６１．５％、６９．２％、９８．１％和２８．８％。②１８例对照标本中相应指标的阳性率分别为１６．７％、１００％、３８．９％、１００％和６１．１％。③比较２组标本，肿瘤组Ｐ５３和Ｂｃｌ２的阳性率显著高于对照组（Ｐ＜０．０１及Ｐ＜０．０５），肿瘤组Ｆａｓ与ＴＵＮＥＬ的阳性率显著低于对照组（Ｐ＜０．０１及Ｐ＜０．０５）。④以上４种蛋白与细胞凋亡存在与否无独立相关性。上述结果表明，在食道癌变中，不仅存在细胞凋亡功能的减低，同时伴有多种凋亡相关基因的表达异常。凋亡功能的异常，可能是多基因共同调控的结果。

By means of immunohistochemistry and TUNEL, the expression of four apoptosisrelated genes and apoptosis ability were examined in situ in human esophageal squamous cell carcinomas. Results: In 52 cases of tumor group, the positive rate (PR) of P53, Fas, Bcl2 and Bax expression was 61.5%, 61.5%, 69.2% and 98.1%,respectively. The PR of apoptotic cell was 28.8%. In 18 cases of control group, the corresponding parameters were 16.7%, 100%, 38.9%, 100% and 61.1%, respectively. As compared with control group, the PR of P53, Bcl2 expression were significantly higher in tumor group (P<0.01 and P<0.05), with the significantly decreased PR of Fas expression and apoptotic cell (P<0.01 and P<0.05). No correlation was showed between each protein expression of four and apoptosis detection. These findings implicate that, during the course of carcinogenesis in esophageal squamous cell carcinomas, the reduction of apoptosis ability is prominent, accompanied with abnormal expression of multiple apoptosisrelated genes