疏花石斛中石斛多糖的提取工艺

Study on Dendrobium Extraction of Polysaccharide from Dendrobium henryi

目的:建立疏花石斛多糖提取优选方案。方法:采用传统水浸提法、纤维素酶法、超声辅助法和微波辅助法4种不同处理方法对疏花石斛多糖进行提取;多糖含量的测定用苯酚-硫酸法。结果:纤维素酶法较优方案为1∶30料液比,50℃,pH 4.5,200 U·mL-1作用2 h;微波辅助法较优方案为料液比1∶30、微波功率档位为中火、作用时间8 min预处理后,80℃浸提1.5 h;超声辅助法较优方案为料液比1∶30、超声功率300 W、超声处理6 min预处理后,80℃浸提1.5 h;传统水浸提法较优方案为料液比1∶30、温度80℃浸提1.5 h。结论:纤维素酶法提取疏花石斛多糖效果最佳,是多糖提取时的首选方案,传统水浸提法宜结合超声辅助法和微波辅助法。

Objective：To establish an optimum method of extraction of polysaccharide from Dendrobium henryi. Method： Tradition water leach, cellulose enzyme, ultrasonic assistant and microwave assistant extracting procedures were used for polysaccharide extraction, and the phenol-sulfuric reaction was used for the determination of polysaccharide. Result：The optimum condition of extraction of Dendrobium polysaccharide was as follows： solid to liguid ratid was 1 ： 30 with enzyme of 200 U ·mL^- 1 extracted for 2 hours at 50 ℃ , pH4.5 in cellulose enzyme extraction ; the third-gears microwave power was used for 8 minutes pretreatment, then extracted at 80 ℃ for 1.5 hours in the microwave assistance extracting; the ultrasonic power 300 W and 6 minutes of pretreatment were used with solid to liquid ratio of 1 ： 30, then extracted at 80 ℃for 1.5 hours in ultrasonic assistance extreacting; and extracted at 80 ℃ for 1.5 hours with solid to liquid ratio of 1 ： 30 in tradition water leach extracting. Conclusion ： The highest yield for Dendrobium polysaccharide extracted occurred in cellulose enzyme extracting, which was the preferred alternative. Tradition water leach extracting should be combined with ultrasonic assistant and microwave assistant extracting.