玉屏风总多糖水提醇沉工艺条件优化

Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng

目的:优化玉屏风总多糖(YTP)的水提醇沉工艺条件。方法:以玉屏风总多糖得率为考察指标,采用正交试验法对YPT水提醇沉工艺的影响因素料液比、提取时间、醇沉浓度进行优化。结果:水提醇沉提取玉屏风总多糖的最佳提取工艺为:料液比1∶12,在90℃下浸提1.5 h,90%乙醇沉淀可使YTP得率达到4.31%。结论:该提取工艺可行,实验结果为确定玉屏风总多糖的水提醇沉工艺提供了实验依据。

Objective：To seek out the optimal water-extracting and alcohol-precipitating technology of total polysaccharides of Yupingfeng （YTP）. Method： Using the yield of YTP as assessment index, three factors were studied by orthogonal design, including ratio of material （g） to extracting solvent （mL）, extracting time and ethanol concentration of precipitating polysaccharide. Result： The yield of YTP was 4.31% by the optimum process conditions listed as follows： ratio of material to extracting solvent was 1： 12, extracting time when the temperature was kept at 90 ℃ as 1.5 h; and the volume fraction of ethanol was 90%. Conclusion： These extraction process is feasible, and the experiment result can provide basic and instruction data for the ascertainment of water extraction technology of YTP.