摘要
目的:采用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD)测定三七止血胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量。方法:以乙腈-水为流动相梯度洗脱(0～12 min,乙腈19%→36%),Hypersil ODS柱(4.0 mm×200mm,5μm)为固定相,流速为1.0 mL·min-1,ELSD参数:漂移管温度45℃,载气流量1.5 L·min-1。结果:三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为97.86%,96.24%和97.58%,RSD分别为1.36%,1.58%和1.13%(n=6)。结论:该方法简便、快速、重现性好,可用于三七止血胶囊的质量控制。
Objective：To develop an HPLC-ELSD method for the content determination of notoginsenoside R1 , ginsenoside RgI and ginsenoside Rb1 in Sanqi Zhixue Capsule. Method： The analysis was carried out on a Hypersil ODS analytical column （4.6 mm × 200 mm, 5 μm） with the mobile phase consisting of acetonitrile-water by gradient elution （0-12 min, acetonitrile 19%→36% ）. The detector drift tube temperature was set at 45℃ and the nitrogen flow rate was 1.5 L·min^-1. Result： The recoveries for notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rh1 were 97.86% , 96.24% and 97.58% with RSD 1.36% , 1.58% arid 1.13% （n = 6） respectively. Conclusion： The developed method is simple, rapid, replicable, and can be used for the quality control of Sanqi Zhixue Capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第1期75-77,共3页
Chinese Journal of Experimental Traditional Medical Formulae
