栽培苦参与野生苦参的药效学研究

Comparative Studies on Pharmacological Effects between Extracts from Cultural and Wild Radix Sophorae Flavescentis

目的:比较栽培与野生苦参的清热、利尿、镇痛方面的药效学作用。方法:以干酵母致大鼠高热模型比较栽培苦参与野生苦参的解热作用;以代谢笼收集大鼠不同时间点的排尿量和总尿量比较栽培与野生苦参的利尿效果;采用热板和扭体法比较观察其对小鼠的镇痛作用。结果:栽培与野生苦参(1,2,4 g.kg-1剂量)均能明显降低酵母菌致热模型大鼠的体温;明显增加大鼠不同时间段的排尿量和排尿总量;栽培与野生苦参3,6 g.kg-1剂量均可明显提高小鼠的热板痛阈值、明显延长小鼠出现扭体的潜伏期并明显减少小鼠的扭体次数,表明其具有非常明显的镇痛作用。结论:栽培苦参与野生苦参都具有明显的清热、利尿和镇痛方面的作用,药效学作用强度比较野生与栽培苦参无显著差异,本实验结果为栽培苦参代替野生苦参提供了实验依据。

Objective： To compare antipyretic, diuretic, anti-tumor and analgesic effects of extracts from cultural and wild Radix Sophorae Flavescentis. Method： The antipyretic action was monitored by dried yeastinduced fever in rats. The diuretic effect was observed in mouse model by collecting and testing urine volumes in different intervals. The analgesic effects were discussed by adopting acetic acid writhing and hot plate test in mice. Result： Both the extracts from cultural and wild Radix Sophorae Flavescentis 1,2,4 g. kg^-1 significantly reduced the fever induced by dried yeast and increased urine volumes in rats at all the intervals. Both of them 3, 6 g·kg^-1 remarkably enhanced the threshold of pain by hot plate, reduced the numbers of writhing and prolonged the latencies of paw licking in mice, showing significant analgesic effects. Conclusion： There is no significant difference in antipyretic, diuretic and analgesic effects between extracts from cultural and wild Sophoraeflavescentis. The results provide experimental evidence for cultural Sophorae flavescentis replacing wild ones in clinic.