无患子皂苷对大鼠心肌缺血的保护作用

Protective Effects of Sapindoside on Experimental Myocardial Ischemia in Rats

目的:观察无患子皂苷对大鼠实验性心肌缺血损伤的保护作用。方法:采用结扎冠脉左前降支的方法复制大鼠心肌缺血模型,将造模成功的大鼠随机均分为模型组、阳性对照组(三七总皂苷0.081 g·kg-1)、无患子皂苷低、中、高剂量组(0.054,0.162,0.486 g·kg-1),每组10只,另取假手术组10只,连续ig给药28 d。分别监测各组大鼠手术前及手术后即刻,1,3,7,10,14,21,28 d的心电图,并测定给药28 d后的心肌梗死率及血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的含量。结果:无患子皂苷各剂量组大鼠心电图S-T段的抬高程度均显著下降,心肌梗死范围明显缩小,血清SOD和GSH-Px的活性显著增强,MDA含量显著降低。结论:无患子皂苷能有效保护大鼠实验性心肌缺血损伤,其作用可能与清除氧自由基,提高抗氧化酶活性及抗脂质过氧化有关。

Objective： To investigate protective effects of sapindoside on experimental myocardial ischemia in rats. Method： Using the method of ligation of left descending coronary artery to establish the rat model of myocardial ischemia. Besides a sham-operated group, successfully-made-model rats were divided into a model group, a positive control group, a low dose, a medium dose and a high dose of sapindoside groups with 10 rats in each group, in which the drug was given by stomach irrigating for consecutive 28 d. ECG of each rat was measured before surgery, immediately, and 1, 3, 7, 10, 14, 21 and 28 d after surgery. The scope of myocardial infarction, the content of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and malonaldehyde （MDA） in each rat serum were measured after the drug was used for 28 d. Result： Within certain dose range of sapindoside, the ECG S-T segment elevation levels in model rats were obviously reduced, the myocardial infarct sizes were decreased, the activities of SOD and GSH-Px in rat serum were enhanced, and the contents of MDA were reduced. Conclusion： sapindoside can effectively protect myocardial ischemic injury of rats. Its role may be related to scavenging oxygen free radicals, enhancing the activity of antioxidant enzyme, and inhibiting lipid peroxidation.