降压颗粒的主要药效学及急性毒性研究

Experimental Study on Main Pharmacodynamics and Acute Toxicity of Jiangya Granules

目的:研究降压颗粒(Jyg)对高血压的药效及安全性。方法:采用肾动脉结扎法建立肾型高血压大鼠模型,实验分为模型组、正常对照组、阳性对照组、降压颗粒高、中、低剂量组(11.4,5.7,2.9 g.kg-1),从而观察其降血压作用;另外,通过显微镜下记录小鼠耳微动脉、微静脉血管管径,观察降压颗粒的扩血管作用。急性毒性实验是以动物能耐受的最大浓度、最大体积的药量1日内2次ig给予小鼠,给药后立刻观察动物反应,并连续观察14 d。结果:降压颗粒3个剂量组均能明显降低肾型高血压大鼠血压,扩张小鼠耳微动脉、微静脉血管,急性毒性实验连续观察14 d后,未发生动物死亡,体重均有所增长,肉眼观察未见明显毒性反应。结论:降压颗粒具有明显的降血压作用,初步证明是安全的。

Objective： To investigate the potency and safety of Jiangya Granules （Jyg）on hypertension. Method： Renal hypertension model was established by ligation of the left renal artery. Healthy rats were divided into a model group, a normal control group, a positive control group, a high dose of Jyg group, a middle dose of Jyg group and a low dose of Jyg group（ 11.4,5.7,2.9 g·kg^-1）to observe the effect of Jyg on lowering blood pressure. In addition, the action of vasodilation was observed by microscope to record vessel diameters of arteriolae and veinules in mice＇ s ears. The experiments in acute toxicity were carried out by oral administration of Jyg to mice given twice a day with tolerable concentration and volume to observe their responses immediately after the administration and continuously for 14 days. Result： All the 3 different dosages of Jyg could significantly depress blood pressure of experimental renal hypertension in rats, and expand the arteriolae and veinules in mice＇ s ears. After 14 days＇ continuous observation, no mouse was found dead. Their weight increased, and no significant toxicity occurred by naked eyes. Conclusion： Jiangya Granule can obviously lower blood pressure, and it is preliminary proven to be safe.