甘草甜素对HepG2.2.15细胞株CCL20的影响

Affection of Glycyrrhizin on Expression of CCL20 in HepG2. 2. 15 Cells

目的:探讨甘草甜素(GL)对HepG2.2.15细胞CCL20表达及细胞存活率的影响。方法:定量RT-PCR检测CCL20的表达,MTT检测细胞的增殖活性,计算细胞存活率,并与空白对照组比较。结果:给药后各组CCL20水平随着剂量的增加而表现为逐渐降低的趋势,与空白对照组比较差异均有统计学意义(P<0.01);MTT实验显示200 mg·L-1以下3个剂量组均可促进细胞增殖(P<0.01);400,800 mg·L-12组均显著抑制细胞增殖(P<0.01)。结论:GL呈剂量依赖抑制CCL20的表达,从而可能抑制HBV引起的免疫炎症反应,但800 mg·L-1组的低表达也可能与细胞的死亡有关。

Objective：To investigate the affection of glycyrrhizin （GL） on the expression of C CL20 mRNA level in HepG2.2. 15 cell line. Method： CCL20 mRNA level was examined by quantitative RT-PCR, cell proliferation was examined by MTT before and after treated with GL, and compared with the control group. Result： The expression of CCL20 was downregulated in GL-treated HepG2.2. 15 cells（P 〈0.01 ）. GL in three groups under 200 mg. L-could promote cell proliferation; 400 mg· L^-1 and 800 mg· L^-1of GL inhibited the growth of cells（P 〈 0.01）. Conclusion： GL inhibited the expression of CCL20 in dose-dependent manner in HepG2.2. 15 cells, and inhibited or promoted cell proliferation.