陕西省克山病病区粮食和人群硒水平调查

Investigation on Selenium levels of food supplies and people in Keshan disease area of Shaanxi Province

目的了解陕西省克山病病区粮食和人群硒水平。方法按整群分层抽样的方法,于2006-2007年,在陕西省克山病区范围内抽取6个病区县（区）的12个自然村作为调查点,采集调查点居民户自产主食粮食小麦和玉米515份,克山病患者和病区健康人发样307份、全血248份及非病区健康人血样64份,用2,3-二氨基萘荧光法、原子荧光法和化学比色法分别测定粮食、发硒、全血硒含量和谷胱甘肽过氧化物酶（GSH-Px）活力。结果病区居民粮食（小麦、玉米）硒含量为（0.045±0.036）和（0.035±0.025）mg/kg,克山病患者发硒含量（0.376±0.091）mg/kg低于病区健康人（0.384±0.077）mg/kg,差异无统计学意义（P〉0.05）;克山病患者全血硒含量（0.071±0.017）mg/L低于病区健康人（0.077±0.017）mg/L,差异有统计学意义（P〈0.05）,病区健康人明显低于非病区健康人（0.090±0.016）mg/L,差异有统计学意义（P〈0.01）;克山病患者GSH-Px（75.16±12.10）U/L低于病区健康人（85.70±14.66）U/L,差异有统计学意义（P〈0.05）。结论病区粮食和人群硒水平明显升高,与生活水平的提高和膳食结构的明显改善有关。

Objective To understand selenium levels of food supplies and people in Keshan disease area of Shaanxi province.Methods From 2006 to 2007,througn stratified cluster sampling,12 villages from 6 endemic counties were randomly selected as investigation points.307 hair samples and 248 whole blood samples from the Keshan patients and healthy people from the same districts,as well as 64 blood samples from other counties were collected and detected by Diaminonaphthalene fluorometric method,Atomic-Fluorescence Spectroscopy and chemical colorimetry.Results The average content of selenium in staple foods（wheat and corn） were 0.045±0.036 mg/kg and 0.035±0.025 mg/kg respectively,the level of hair selenium was 0.376±0.091 mg/kg,lower than the healthy people（0.384 ±0.077）mg/kg,with nonsignificant difference（P〉0.05）;The average content of whole blood selenium 0.071±0.017 mg/L was lower than healthy people from the same counties 0.077±0.017 mg/L,with significant difference（P〈0.05）,and the average content of whole blood selenium of healthy people in endemic counties was lower than the healthy in non endemic counties 0.090±0.016 mg/L,with significant difference（P〈0.01）;The average contents of GSH-Px of Keshan patients 75.16±12.10 U/L ere lower than the healthy people from the same counties 85.70±14.66 U/L,with significant different（P〈0.05）.Conclusions The average contents of selenium in staple foods and people gradually increased,and this has good relationship with living standard increasing and dietary pattern improving noticeably.