儿茶素对人肝癌细胞HepG2的影响

Effects of Catechin on human hepatocellular carcinoma cell line HepG2

目的研究儿茶素[（＋）-catechin,Cat]不同时间、不同浓度对人肝癌细胞（HepG2）的增殖、迁移能力的抑制及诱导凋亡作用。方法 HepG2细胞分别与不同浓度Cat作用,MTT法检测细胞增殖率的变化,显微镜观察细胞形态学变化,划痕法检测细胞的迁移能力,流式细胞术检测细胞凋亡率,免疫组织化学染色法检测HepG2的Bcl-2、Bax和Caspase-3蛋白表达。结果 Cat（4-100mg·mL^-1）能明显抑制人肝癌细胞HepG2的增殖和迁移能力,并呈浓度依赖性。流式细胞术检测结果显示Cat（4mg·mL^-1）作用于HepG2细胞24h即可诱导细胞凋亡。免疫组织化学结果显示Cat（4-20mg·mL^-1）的Bax和Caspase-3的表达呈浓度依赖性升高,而Bcl-2的表达呈浓度依赖性降低。结论 Cat可以一定程度的抑制HepG2细胞的增殖和迁移能力并诱导其凋亡,其诱导凋亡机制可能与其下调HepG2细胞内Bcl-2蛋白的表达,上调Bax蛋白的表达,从而促进Caspase-3活化有关。

Aim To investigate the effects of Catechin（Cat）on proliferation,migration and apoptosis of human hepatocellular carcinoma cell line HepG2.Methods HepG2 cells were incubated with different concentrations of Catechin.The proliferation inhibition of HepG2 was detected by MTT assay.Morphology of cells was observed with phase contrast microscope.Cell migration was measured by wound healing assay.Cell apoptotic rate was evaluated using flow cytometry.The expressions of apoptosis-related protein Bcl-2,Bax and Caspase-3 were measured by immunohistochemical staining.Results Treatment with Catechin（4-100 mg·L-1）had a concentration-dependent inhibitory effect on cell proliferation and migration.Furthermore,the results of flow cytometry revealed that Catechin（4 mg·L-1）induced the cell apoptosis which was associated with inhibiting the expression of Bcl-2 as well as increasing expression of Bax and Caspase-3 in a concentration-dependent manner.Conclusion Catechin has proliferation-inhibitory and pro-apoptosis effects on the HepG2 cells,which may be related to the down-regulation of Bcl-2,the up-regulation of Bax,and the activation of Caspase-3.