红车轴草异黄酮对维甲酸致小鼠骨质疏松的预防作用

Preventive effect of trifolium pratense isoflavones on retinoic acid induced osteoporosis in mice

目的研究红车轴草异黄酮对维甲酸致小鼠骨质疏松的预防作用,并探讨其主要作用机制。方法维甲酸(90mg.kg-1)致小鼠骨质疏松模型,红车轴草异黄酮(50、100、200mg·kg-1)灌胃给药,实验过程监测体重,2周后以比色法测定血清钙、磷含量和碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)活性,竞争放射免疫法测定血清中骨钙素(BGP)和雌二醇(E2)含量,取股骨进行骨组织形态学观察。结果维甲酸可引起高转换型骨质疏松,红车轴草异黄酮可升高E2值,降低P、ALP、TRAP、BGP水平。结论红车轴草异黄酮对维甲酸致小鼠骨质疏松有一定的预防作用,其机制可能与其减缓雌激素水平降低,抑制骨高转换有关。

s：Aim To observe the preventive effect of trifolium pratense isoflavones（TPIF）on retinoic acid induced osteoporosis in mice.Method The mouse osteoporosis was induced by given retinoic acid intragastrically for two weeks.The histomorphological features of bone were observed and biochemical indexes in serum（Ca,P,ALP,TRAP,E2,BGP）were determined after the mouse were given TPIF at the dose of 50,100,200 mg·kg^-1 respectively.Results Trifolium pratense isoflavones could induce high turnover of osteoporosis.The levels of P,ALP,TRAP and BGP in the TPIF groups were lower than the model group,while the E2 level was higher than the model group.Conclusions Trifolium pratense isoflavones can prevent the loss bone in the experimental mice.The machanism may be that it improves the level of estrogenic hormone and inhibits the high bone turnover.