通心络和人参总皂苷对过度疲劳致血管内皮功能障碍大鼠主动脉CPT-I和AMPKα表达的干预作用

The effect of Tongxinluo and ginsenosides on the expression of CPT-I and AMPKα in aorta of rats with endothelial dysfunction caused by excessive fatigue

目的观察通心络和人参总皂苷对过度疲劳致血管内皮功能障碍大鼠主动脉CPT-I和AMPKα表达的干预作用。方法采用"基础饮食+负重游泳法"建立疲劳应激动物模型,给予通心络和人参总皂苷进行干预,观察大鼠一般状况、主动脉内皮细胞形态和结构,血中ET-1和NO水平,采用Real-Time PCR检测主动脉CPT-I、AMPKαmRNA表达,Western blot检测主动脉CPT-I、AMPKα、p-AMPKα蛋白表达。结果过度疲劳应激可导致血管内皮结构和分泌功能损伤,同时CPT-I、AMPKαmRNA和蛋白表达降低;通心络和人参总皂苷可保护血管内皮结构和功能完整性,升高CPT-I、AMPKαmRNA和CPT-I、AMPKα、p-AMPKα蛋白表达。结论通心络和人参总皂苷可通过增强血管AMPK mRNA基因和蛋白表达及p-AMPKα蛋白表达,进而调控血管局部与脂质β氧化相关的CPT-I mRNA和蛋白表达,从而对过度疲劳大鼠血管内皮发挥保护作用。

Aim To investigate the effect of Tongxinluo and Ginsenosides on the expression of CPT-I and AMPKα in aorta of rats with endothelial dysfunction caused by excessive fatigue.Methods Excessive fatigue rat models were built by basic diet and compelled weight-loading swimming,and treatment with Tongxinluo capsule and Ginsenosides.The general condition,morphological changes of endothelial cell in aorta,the level of ET-1 and NO in blood were detected.The mRNA expression of CPT-I and AMPKα in aorta were detected by real-time reverse transcription PCR.The protein expression of CPT-I,AMPKα and p-AMPKα in aorta were detected by Western blot.Results The damage of the structure and function of endothelial cell was induced by excessive fatigue.Meanwhile,the mRNA and protein expression of CPT-I,AMPKα decreased.Tongxinluo and Ginsenosides could protect the structure and function of endothelial cell,increase the mRNA expression of CPT-I,AMPKα and increase the protein expression of CPT-I,AMPKα,p-AMPKα.Conclusions Tongxinluo and Ginsenosides can increase the mRNA and protein expression of AMPKα and increase the protein expression of p-AMPKα,then regulate the mRNA and protein expression of CPT-I which is related to β of lipid oxidation in aorta,and produce the protective effect on endothelial cell of excessive fatigue rats.