PRDM14在肺癌细胞系中的表达

Expression and distribution of PRDM14 mRNA and protein in lung cancer cell lines

目的检测PRDM14在肺癌细胞系中的表达及分布情况。方法采用RT-PCR技术和免疫荧光方法分别检测人支气管上皮细胞系(HBE)和6个肺癌细胞系中PRDM14mRNA和蛋白的表达。结果 RT-PCR结果显示PRDM14与GAPDH电泳条带的平均光密度比值在肺癌细胞系中显著高于HBE细胞系(P<0.001);免疫荧光结果显示PRDM14蛋白表达的平均荧光强度在肺癌细胞系中高于HBE细胞系(P<0.05),PRDM14蛋白荧光信号主要定位于细胞浆。结论 PRDM14在HBE细胞系低表达,在肺癌细胞系高表达,主要分布于细胞浆,PRDM14可能在肺癌的发生发展中发挥作用。

Objective To detect the expression and distribution of positive regulatory domain zinc finger protein 14（PRDM14） in lung cancer cell lines. Methods The expression of PRDM14 mRNA and protein was assayed in human normal bronchi epithelium（HBE） and six lung cancer cell lines using by RT-PCR and immunofluorescence respectively. Results The average optical density（AOD） ratio for PRDM14 mRNA and GAPDH mRNA was much higher in lung cancer cell lines than in HBE cell line by RT-PCR（P〈0.001）. The AOD values of PRDM14 protein were upregulated in the cells of lung cancer cell lines than in HBE cell lines by immunofluorescence（P〈0.05）. Conclusion Overexpression of PRDM14 in lung cancer cell lines than in HBE cell line indicates that PRDM14 might play an important role in the development of lung cancer.