rhG-CSF对2.3Gy中子照射比格犬凝血功能的影响

Effect of rhG-CSF on Blood Coagulation in Beagles Irradiated by 2.3 Gy Neutron

本研究旨在观察rhG-CSF对2.3Gy中子照射比格犬凝血功能的影响,以期为中子照射所致凝血功能紊乱提供新的治疗措施。给予10只成年比格犬2.3Gy中子照射,动物随机分为对症治疗组和rhG-CSF治疗组。rhG-CSF治疗组动物自照射当天开始给予rhG-CSF10μg/kg,每天1次皮下注射,连续给药21天。分别在照射后不同时间抽取外周血检测血小板数,血小板体外聚集实验、血栓弹力图描记分法(TEG)、凝血四项法检测两组动物的凝血情况。结果表明,2.3Gy中子照射后,比格犬血小板数量和聚集功能同时下降;凝血系统在照射后初期处于高凝状态,极期处于低凝状态;凝血功能改变以内源性凝血途径为主;在病程初期和极期纤维蛋白原含量均明显增高。rhG-CSF治疗可以纠正2.3Gy中子照射初期的高凝状态,缩短极期凝血活酶生成时间,加快血凝块形成的速度,下调纤维蛋白原的异常增高,改善血小板聚集功能。结论:rhG-CSF可改善照射所致的凝血功能紊乱。

The aim of this study was to investigate the effect of recombinant human granulocyte stimulating factor （rhG-CSF） on blood coagulation of beagles irradiated by 2.3 Gy neutron so as to provide new therapy for blood coagulation disorder after neutron irradiation. 10 beagles were exposed to 2.3 Gy neutron, and then randomly assigned into supportive care group and rhG-CSF-treated group. The rhG-CSF-treated cohorts were injected subcutaneously with rhG-CSF （10 μg/kg·d） beginning at the day of exposure for 21 consecutive days. Peripheral blood platelet counts were examined once every two days. In vitro platelet aggregation test, thromboelastography and blood clotting tetrachoric tests were also performed. The results indicated that the blood clotting system of irrediated dogs was in hypercoagulabale state in the early days after 2.3 Gy neutron irradiation, and became hypocoagulable at crisis later and were mainly on intrinsic coagulation pathway. Blood fibrinogen increased markedly during the course of disease, while platelet counts and aggregation function were decreased remarkably. rhG-CSF administered daily could correct hypercoagulable state induced by 2.3 Gy neutron irradiation at the early time post exposure, shortened the thromboplatin generation time and clotting formation, down-regulated the abnormal high fibrinogen in blood, and improved platelet aggregation function. It is concluded that rhG-CSF can improve coagulation disorders of irradiated dogs.