摘要
本研究探讨内吗啡肽-1对正常人骨髓基质细胞表面黏附分子细胞间黏附分子(ICAM-1)和血管细胞黏附分子(VCAM-1),以及基质细胞分泌的细胞因子IL-6、TNF-α的调控作用。应用流式细胞术检测黏附分子ICAM-1和VCAM-1的表达,用ELISA法检测细胞因子IL-6、TNF-α的浓度。结果表明:加入内吗啡肽-1培养24小时后ICAM-1和VCAM-1表达与对照组比较有显著性差异(p<0.05),但内吗啡肽-1浓度的改变对于二者的表达无影响;IL-6、TNF-α的浓度实验各组数据与对照组比较无显著性差异(p>0.05)。结论:内吗啡肽-1能调控正常骨髓基质表面ICAM-1和VCAM-1的表达;对于正常骨髓基质细胞分泌的细胞因子IL-6、TNF-α无促进或抑制作用。
The aim of study was to investigate the regulatory effect of endomorphin-1(EM-1) on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours (p0.05), and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no diffe-rence in secretion of IL-6 and TNF-α between experimental and control groups (p0.05). It is concluded that endo-morphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第6期1574-1578,共5页
Journal of Experimental Hematology
基金
甘肃省科技攻关项目
编号:2GS042-A43-014-19
关键词
内吗啡肽-1
骨髓基质细胞
造血调控
endomorphin-1
bone marrow stroma cell
hematopoietic regulation