期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

沉默GRP78基因对人卵巢癌SKOV3细胞侵袭力的抑制作用 被引量：1

下载PDF
导出
摘要 目的探讨RNA干涉技术(RNAi)沉默葡萄糖调节蛋白78(GRP78)基因对人卵巢癌SKOV3细胞侵袭力的影响,阐明GRP78基因沉默对SKOV3细胞侵袭力抑制作用的生物学机制。方法设计并构建pSilencerTM3.0-H1-GRP78 siRNA重组质粒,脂质体介导转染至SKOV3细胞。RT-PCR和Western印迹法检测GRP78基因表达;RT-PCR检测MMP-2、MMP-9 mRNA表达;Transwell小室侵袭实验检测细胞侵袭力;Transwell小室迁移实验检测细胞迁移率。结果 RT-PCR及W estern印迹检测转染GRP78 siRNA重组质粒SKOV3细胞GRP78基因表达抑制;RT-PCR结果显示与对照组相比转染GRP78 siRNA重组质粒SKOV3细胞MMP-2、MMP-9 mRNA表达降低;Transwell小室侵袭实验和迁移实验结果显示转染GRP78 siRNA重组质粒SKOV3细胞穿透细胞数(P<0.05)和迁移率均明显降低(P<0.05)。结论①转染GRP78 siRNA重组质粒有效抑制SKOV3细胞GRP78基因表达;②抑制GRP78基因表达能降低SKOV3细胞的侵袭力和迁移力,有望为抑制卵巢癌转移基因治疗提供新的靶点。
作者 范丽梅 魏敏 杨淑丽 崔松花 崔满华 张丽波
机构地区 吉林大学第二医院妇产科 通榆县生殖保健院电诊科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第23期3516-3519,共4页 Chinese Journal of Gerontology
基金 吉林省科技发展计划项目(2007052627)
关键词 葡萄糖调节蛋白78 人卵巢癌SKOV3细胞 RNA干扰 侵袭
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献15

  • 1Hata K,Nakayama K,Fujiwaki R,et al.Expression of the angopoietin-1,angopoietin-2,Tie-2 and vascular endotheial growth factor gene in epithelial ovarian cancer[J].Gynecol Oncol,2004;93(1):215-22.
  • 2Lee AS.The glucose-regulated proteins:stress induction and clinical applications[J].Trends Biochem Sci,2001;26(8):504-10.
  • 3Jamora C,Dennert G,Lee AS,et al.Inhibition of tumor progression by suppression of stress protein GRP78/BiP pinduction in fibrosarcoma B/C 10ME[J].Proc Natl Acad Sci USA,1996;93(15):7690-4.
  • 4Fu Y,Lee AS.Glucose regulated proteins in cancer progression,drug resistance and immunotherapy[J].Cancer Biol Ther,2006;5(7):741-4.
  • 5Zhang J,Jiang Y,Jia Z,et al.Association of elevated GRP78 expression with increased lymph node metastasis and poor prognosis in patients with gastric cancer[J].Clin Exp Metastasis,2006;23(7-8):401-410.
  • 6Gonzalez-Gronow M,Cuchacovich M,Llanos C,et al.Prostate cancer cell proliferation in vitro is modulated by antibodies against glucose-regulated protein78 isolated from patient serum[J].Cancer Res,2006;66(23):11424-31.
  • 7Dong D,Ni M,Li J,et al.Critical role of the stress chaperone GRP78/BiP in tumor proliferation,survival,and tumor angiogenesis in transgene-induced mammary tumor development[J].Cancer Res,2008;68(2):498-505.
  • 8Yang GH,Li S,Pestka JJ,et al.Down-regulation of the endoplasmic reticulum chaperone GRP78/BiP by vomitoxin (Deoxynivalenol)[J].Toxicol Appl Phamacol,2000;162(3):207-17.
  • 9Miliner J.RNA interference for treating cancers caused by viral infection[J].Expert Opin Biol Ther,2003;3(3):459-67.
  • 10苏荣健,李贞,程留芳,魏嘉,李宏丹.特异性下调葡萄糖调节蛋白78表达对肝细胞癌侵袭和转移能力的影响[J].细胞生物学杂志,2007,29(6):889-894. 被引量：15

二级参考文献21

  • 1Jonbs JL, Walker RA. Control of matrix metalloproteinase activity in cancer [J]. J Pathoh 1997, 183:377 379.
  • 2Stetler-Stevenson WG, Aznavoorian S, Liotta LA, Tumor cell interactions with the extracellular matrix during invasion and metastasis [J]. Annu Rev Cell Biol, 1993, 9: 541-573.
  • 3Turpeenniemi-Hujanen T, Gelatinases (MMP-2 and 9) and their natural inhibitors as prognostic indicators in solid cancers [J]. Biochimie, 2005, 87 (3-4): 287-297.
  • 4Zucker S, Lysik RM, Zarrabi MH, et al. M (r) 92, 000 type IV collagenase is increased in plasma of patients with colon cancer and breast cancer [J]. Cancer Res, 1993, 53 (1): 140 -146.
  • 5Kim TD, Song KS, Li G, et al. Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer [J]. BMC Cancer, 2006, 18: 6: 211.
  • 6Nagel H, Laskawi R, Wahlers A, et al. Expression of matrix metalloproteinases MMP-2, MMP-9 and their tissue inhibitors TIMP-1,-2, and -3 in benign and malignant tumours of the salivary gland [J]. Histopathology, 2004, 44 (3): 222-231.
  • 7Hoikkala S, Paakko P, Soini Y, et ah Tissue MMP-2 and MMP-9 [corrected] are better prognostic factors than serum MMP-2/TIMP-2--complex or TIMP-1 [corrected] in stage [corrected] I -- Ⅲ lung carcinoma [J]. Cancer Lett, 2006, 236 (1): 125-132.
  • 8Bown PD. Matrix metalloproteinase inhibitors: a novel class of anticancer agents [J]. Adv Enzyme Regul, 1995, 35: 293-241.
  • 9Fujii T et al. J Hepatology, 2004, 41:1041
  • 10Vincent-Salomon A et al. Breast Cancer Res, 2003, 5:1011

共引文献18

同被引文献12

引证文献1

二级引证文献2

中国老年学杂志
2010年 第23期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部