罗格列酮对THP-1源性泡沫细胞ABCA1、Caveolin-1蛋白水平的影响

Effect of rosiglitazone on protein ABCA1 and Caveolin-1 in THP-1-derived foams

目的观察罗格列酮干预THP-1源性泡沫细胞腺苷三磷酸结合盒转运体A1(ABCA1)蛋白、小凹蛋白-1(Caveolin-1蛋白)表达变化,以探讨罗格列酮防治动脉粥样硬化(AS)的可能机制。方法培养THP-1细胞,佛波脂(PAM)使之巨噬化,氧化型低密度脂蛋白(ox-LDL)使巨噬细胞形成泡沫细胞,罗格列酮和泡沫细胞孵育48 h,用流式细胞技术检测ABCA1蛋白、Caveolin-1蛋白的水平。结果①ox-LDL处理巨噬细胞后,经油红O染色阳性细胞百分数增加(P<0.05),罗格列酮可显著缓解ox-LDL所致的泡沫细胞阳性数(P<0.05)。②罗格列酮组均增加ABCA1蛋白、Caveolin-1蛋白的表达,与泡沫细胞组相比,均有显著性差异(P<0.05)。结论 ABCA1、Caveolin-1蛋白的增加可能是罗格列酮抗AS的机制之一。

Objective To study the effect of rosiglitazone on ATP Binding Cassette Transporter A1（ABCA1） and Caveolin-1 and explore the probable mechanism.Methods THP-1 cells were cultured.Macrophages were induced from THP-1 cell by PMA.Models of foam cells were built by incubating macrophages with ox-LDL.Foam cells were incubated with rosiglitazone for 48 h.The expressions of protein ABCA1 and Caveolin-1 were determined by flow cytometry.Results Macrophages incubated with ox-LDL formed into foam cells.Rosiglitazone could decrease the members of the foam cells and increase the expressions of protein ABCA1 and Caveolin-1（P0.05）,compared with control group.Conclusions Rosiglitazone can increase the expressions of protein ABCA1 and Caveolin-1,which may be one of mechanisms of rosiglitazone to treat atherosclerosis.