摘要
本实验通过SDS-PAGE技术,对陇东地区部分冬小麦品种(系)的高分子量谷蛋白亚基组成及亚基组成频率进行了分析。结果表明陇东地区冬小麦品种(系)存在16种亚基组合类型,以N,7+8,2+12亚基含量丰富,其出现频率占品种(系)总数的36.50%,而含5+10亚基的亚基组合类型只有3种,仅占品种(系)数量的9.62%。Glu-1位点遗传多样性单一,Glu-A1位点仅有2种等位变异形式,N亚基频率最高(71.15%);Glu-B1位点有4种等位基因变异形式,7+8亚基频率最高(59.62%);Glu-D1位点上有6种等位基因变异形式,2+12亚基频率最高(61.54%)。通过对地方品种、育成品种(系)和引进品种HMW-GS组成比较发现,地方品种亚基的组成类型呈现出组成单一,优质亚基少的特点;而外引品种和育成品种亚基类型较为丰富,且包含一定数量的优质亚基。
High molecular weight glutenin subunit composition and frequencies of some varieties in Longdong were analyzed by SDS-PAGE techniques.The results showed that there were 16 HMW-GS subunits combinations in tested materials.The subunits of N,7＋8,2＋12 were major type account for 36.50% of whole varieties.But there were only 3 subunits combinations of 5＋10 subunit,account for 9.62% of whole varieties.HMW-GS subunit composition characteristics and frequency showed that there was lower genetic diversity in Glu-1 locus,Glu-D1 locus encoded 6 allelic variations,and the frequency of N subunit was the highest（71.15%）.Glu-B1 locus encoded four allelic variations,the frequency of 7＋8 was the highest（59.62%）.Glu-A1 locus encoded 2 allelic variations,and the frequency of 2＋12 was the highest（61.4%）.Subunits combinations of the landrace variety presented the characteristics of simple composition and lacked high quality subunits.But the modern variety and introduced variety had comparatively abundant subunits combinations which contained certain high quality suunits.
出处
《种子》
CSCD
北大核心
2010年第11期42-46,50,共6页
Seed
基金
甘肃省农业厅生物技术专项(GNSW-2009-23)
陇东学院青年科技基金(XY2K0803)
