纳米硒对昆明系小鼠的急性毒性及蓄积毒性试验

Nano-Se acute toxicity and accumulative tests for Kunming mice

本试验用昆明系小鼠作为试验动物分别进行急性毒性试验、蓄积毒性试验以评价粒度为100 nm纳米硒的毒性。急性毒性试验灌注浓度分别为482.04 mg·kg^-1、303.94 mg·kg^-1、191.64 mg·kg^-1、20.83 mg·kg^-1、76.19 mg·kg^-1,根据小鼠死亡率确定纳米硒的LD50。在急性毒性试验的基础上,采用1/5 LD50固定剂量染毒法进行蓄积毒性试验。试验结果表明,纳米硒毒性导致小鼠死亡主要集中在灌注后48 h内,一次灌注损伤在灌注后第5天可得到恢复,纳米硒LD50为303.94mg/kg体重（276.75-391.66 mg/kg体重）;纳米硒在成年昆明系小鼠体内的蓄积系数K〉5。

Acute toxicity test and accumulative test were conducted to assess potential toxicity of nano-Se particles in sizes of 100 nm.Improved Korbor method was adopted to conduct acute toxicity test,dosage gradients of nano-Se preparation with 0.25 percent carboxymethyl cellulose solution were determined according to lethal dose and no mortality dose of nano-Se preparation which were concluded from pre-experiment.Dosage gradients of nano-Se preparation were orally administered 482.04,303.94,191.64,120.83,76.19 mg se/kg bw.Median lethal does（LD50） and pathological transform of acute poisoning mice were determined by mortality of mice in different groups during 21 days.One fifth of LD50 as dosage administered orally was adopted for 25 days in accumulative test.It could be concluded from acute toxicity that LD50 for nano-Se was 303.94 mg/kg BW（276.75～391.66 mg/kg bw） and toxicity of nano-Se lethal dosage could resulted in death of mice within 48h,it took 5 days to recover from acute toxicity at non lethal dosage.Results of accumulative test showed that K5 which was a slight accumulation.