大负荷运动对大鼠心肌细胞膜钠钾泵、钙泵与肌浆网钙泵活性的影响

Effects of Heavy Load Training on Myocardial Cellular Membrane Na^+-K^+-ATPase,Ca^(2+)-ATPase Activity and Sarcop-lasmic Rreticulum Ca^(2+)-ATPase Activity in Rat

目的:探讨大负荷跑台运动对心肌细胞膜Na+-K+-ATPase、Ca2+-ATPase与肌浆网Ca2+-ATPase活性的影响及其与超微结构变化的关系。方法:成年雄性SD大鼠24只,随机分为空白对照组(A组)、运动后即刻组(B组)与运动后24h组(C组)。定磷法测定各组大鼠心室肌细胞膜Na+-K+-ATPase、Ca2+-ATPase活性与肌浆网Ca2+-ATPase活性;电镜观察各组大鼠左室前壁心肌细胞超微结构变化。结果:B组心肌细胞膜Na+-K+-ATPase、Ca2+-ATPase活性显著低于其他两组,C组肌浆网Ca2+-ATPase活性显著低于其他两组;B组线粒体结构明显损伤,C组肌原纤维结构明显损伤、肌细胞及肌浆网肿胀。结论:大负荷运动引起大鼠心肌细胞膜Na+-K+-ATPase、Ca2+-ATPase与肌浆网Ca2+-ATPase活性降低及心肌超微结构异常,但变化不完全同步;细胞膜Na+-K+-ATPase、Ca2+-ATPas活性下降可能与线粒体大面积损伤有关。

The purpose of this study was to explore the effects on myocardial cellular membrane Na＋-K＋-ATPase,Ca2＋-ATPase activity,and sarcoplasmic reticulum Ca2＋-ATPase activity of rats after once high intensive exercise on treadmill,and explore the relationship between that and myocardial ultrastructure.24 grown male SD rats were divided into normal contrapositive group（group A）,instantly after exercise group（group B）and 24 hour after exercise group（group C）.The activity of ventricular myocardial cellular membrane Na＋-K＋-ATPase,Ca2＋-ATPase and sarcoplasmic reticulum Ca2＋-ATPase were determined using the method of phosphorus,the left ventricular antetheca cardiac muscle cell ultrastructure was observed under Transmission Electron Microscope（TEM）.The result showed that the myocardial cellular membrane Na＋-K＋-ATPase Ca2＋-ATPase activity of group B was significant lower than other two groups,the sarcoplasmic reticulum Ca2＋-ATPase activity of group C was significant lower than other two groups.Myocardial mitochondrial significant damage of group B was observed under TEM,and myofibril significant damage,cell sarcoplasmic reticulum swelling of group C.It concludes that heavy load training may induce myocardial cellular membrane Na＋-K＋-ATPase,Ca2＋-ATPase activity and sarcoplasmic reticulum Ca2＋-ATPase activity decreasing,also induce ultrastructure to damage,but no completely in sync.It also hints that there might be some certain relationship between lowing of cellular membrane Na＋-K＋-ATPase,Ca2＋-ATPase activity and the large area injury of mitochondrial.