染色纺织品中禁用偶氮染料的高效液相色谱-电喷雾电离质谱联用法测定

Determination of banned AZO colourants in dyed textiles by liquid chromatography-electrospray ionization mass spectrometry

建立了采用高效液相色谱电喷雾电离质谱法(HPLC-ESI/MS)测定纯毛精纺花呢中残留偶氮染料的方法。纯毛精纺花呢试样中禁用偶氮染料经提取和还原后,在Eclipse XDB-C18柱(150 mm×2.1 mm,I.D.,5μm)上采用甲醇/醋酸醋酸铵溶液(5 mmol/L,pH=5.5)为流动相进行梯度洗脱分离,采用电喷雾电离离子化技术在选择离子监测(SIM)模式下进行测定,外标法定量。实现了21种芳香胺的同时分离检测,其加标回收率为80.0%～102.4%,相对标准偏差小于9.4%。该方法简便、快速、灵敏,能满足纺织品中残留物的检测需求。

A method has been developed for the determination of the banned AZO colourants in dyed pure worsted fabrics by high-performance liquid chromatography coupled with an electrospray ionization mass spectrometry（HPLC-ESI/MS）.After the banned AZO colourants in dyed worsted fabrics were extracted and deoxidized,the separation was performed on a ZORBAX Eclipse XDB-C18 column（150 mm × 2.1 mm I.D.,5 μm）with mobile phase consisting of 5 mmol/L aqueous ammonium acetate-acetic acid /methanol in gradient elution.Detection was carried out by an ion trap mass spectrograph with an ESI interface in the positive mode using external standard method.Simultaneous separation and determination of 21 aromatic amines were realized.The recoveries were between 80.0% ～ 102.4 %,and the RSDs were less than 9.4%.This method was found to be sensitive,fast and accurate,and it can be used for the determination of the banned AZO colourants in dyed textiles samples.