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新疆地区荧光原位杂交产前诊断非整倍体的应用价值 被引量：3

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摘要 目的探讨荧光原位杂交(FISH)技术产前诊断常见染色体非整倍体的应用价值。方法采用21、18、13、和Y染色体特异性DNA探针对96例孕18～23周孕妇的未培养羊水细胞进行FISH检测,同时行羊水细胞染色体核型分析。结果 FISH检测98例成功96例(97.9%),其中染色体数目正常93例(48例为46,XX;45例为46,XY)染色体数目异常3例(2例47,XX,+21;1例47,XX+18)。FISH诊断结果与核型分析结果一致,染色体数目异常的3例与传统核型分析结果完全一致。结论 FISH技术用于产前诊断常见染色体非整倍体,具有简便、快速、特异性强敏感性高、所用样本量少等优点。
作者 刘鸿春 王瑞 黄国香 杨柳
机构地区 兰州军区乌鲁木齐总医院临床医学研究所
出处 《中国优生与遗传杂志》 2010年第12期42-43,共2页 Chinese Journal of Birth Health & Heredity
关键词 荧光原位杂交 产前诊断 非整倍性 羊水细胞 DNA探针
分类号 R714.55 [医药卫生—妇产科学]
  • 相关文献

参考文献6

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二级参考文献27

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共引文献13

同被引文献19

  • 1Caine A, Maltby AE, Parkin CA, et al. Prenatal detection of Downrs syndrome by rapid aneuploidy testing for chromosomes 13,18,and 21 by FISH or PCR without a full karyotype:a cyto genetic risk assessment[J]. Lancet, 2005,366 (9480) : 123 -128.
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  • 9沈艳艳,李健,孔辉,吴慧南,吴琼,葛运生,黄新力,周裕林.荧光原位杂交技术在复杂染色体异常产前诊断中的应用[J].中华医学遗传学杂志,2009,26(5):529-532. 被引量：9
  • 10刘晗,廖灿,黄以宁,潘敏,易翠兴,袁思敏,胡舜妍,钟惠珠.应用优化荧光原位杂交技术诊断110例羊水间期细胞常见非整倍体[J].中华医学遗传学杂志,2010,27(4):453-456. 被引量：13

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中国优生与遗传杂志
2010年 第12期

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