6个家兔群体KAP6.1基因的遗传多样性分析

Genetic diversity of KAP6.1 gene in 6 rabbit populations

设计了2对特异性引物,经过序列拼接,获得了家兔KAP6.1基因的全长CDS序列,并采用PCR-SSCP方法对6个家兔群体KAP6.1基因的全长CDS序列进行分析.结果显示,KAP6.1-1座位发现了2个等位基因,3种基因型,且A755G突变处于该基因的增强子区域,而KAP6.1-2座位未发现突变位点;6个家兔群体均处于哈代-温伯格平衡,表现为中度或低度多态;福建黑兔与九嶷山兔、有色獭兔与白色獭兔、皖系长毛兔与苏系长毛兔的不同基因型分布差异不显著(P>0.05),而其他家兔群体彼此间的不同基因型分布存在显著(P<0.05)或极显著差异(P<0.01).

Full-length CDS of KAP6.1 gene was obtained by sequence assembly with 2 specific primers.Then the sequences in 6 rabbit populations were scanned by PCR-SSCP.The results showed that 3 genotypes and 2 alleles were found at KAP6.1-1 loci,as well as the A 755 G mutation which was located at the enhancer region,while no mutation was found at KAP6.1-2 loci.The distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium and presented either intermediate level or low level of diversity;and its difference was either significant（P0.05） or very significantly（P 0.01） from one another populations except between Fujian black rabbit and Jiuyishan rabbit,chinchilla and white rex rabbit,wan-line and su-line angora rabbit（P0.05）.