茶尺蠖核型多角体病毒湖北分离株的分子鉴定

Molecular identification of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains

运用PCR技术扩增茶尺蠖核型多角体病毒安徽分离株(EcobNPV-AH)和湖北分离株(EcobNPV-HB)的同源重复序列2(hr2),并对湖北分离株的hr2进行克隆和序列测定.结果表明,茶尺蠖核型多角体病毒安徽分离株hr2扩增产物为2条DNA片段,大小约1300bp和1800bp,湖北分离株的hr2扩增产物为1条DNA片段,大小约1800bp,由基因型组成判断,EcobNPV-HB是新的地区分离株,其hr2全长1761个核苷酸,GC含量21.7%,编码540个氨基酸,具有重复性回文序列,较GenBank中登录的EcobNPV-AH的hr2多362个核苷酸.

Homologous repeat region 2（hr2） of Ectropis obliqua nucleopolyhedrovirus（EcobNPV） isolated from Hubei and Anhui strains were amplified by PCR.The results showed that only one 1.8 kb DNA fragment was amplified from EcobNPV-HB strains and two DNA fragments was amplified from EcobNPV-AH strains,which suggested that EcobNPV-HB strains was an independent strains different from EcobNPV-AH strains.hr2 of Ectropis obliqua nucleopolyhedrovirus isolated from Hubei strains was cloned into pMD18-T vector,sequenced and analyzed.The hr2 of EcobNPV-HB strains was composed of 1 761 nucleotides and coded 540 amino acids with a G＋C content of 21.7%,comprised of perfect palindromes.Extra 362 nucleotides were observed compared with hr2 of EcobNPV-AH strains in GenBank.