脑缺血致痴呆大鼠海马神经元的病理改变

Cerebral ischemia led to pathological changes of hippocampal neurons in dementia rat

目的:探讨双侧颈总动脉永久性结扎后脑缺血致大鼠海马神经元的病理改变。方法:将30只大鼠随机分为2组,每组15只。实验组:大鼠用水合氯醛(350mg/kg)腹腔注射麻醉后,双侧颈总动脉永久性结扎(2-VO)。对照组:与实验组做同样手术,但不结扎双侧颈总动脉。造模后第7d、21d后,取出脑组织,HE染色,观察大鼠海马神经元的病理改变。结果:对照组7d及21d镜下海马区神经元排列规则,无明显神经元变性、液化性坏死及神经胶质细胞增生。模型组7d后海马区神经元排列不规则,少量神经元胞核固缩、溶解或消失,可见点状液化性坏死及空泡形成,神经元数量减少,神经胶质细胞少量增生。模型组21d,海马区神经元排列稀疏,胞核固缩、溶解或消失,可见点状液化性坏死,有较多空泡形成,神经元数量减少,神经胶质细胞少量增生。结论:大鼠双侧颈总动脉结扎能较好地模拟人类血管痴呆(VD)的发病机制,并从形态学上提供了VD患者海马区锥体细胞出现变性、死亡、丢失的证据。

Objective：To study Cerebral ischemia led to pathological changes of hippocampal neurons in dementia rat ,After permanent bilateral common carotid artery ligated. Methods：30 rats were randomly divided into two groups of 15 rats. Experimental group： After the rats were anesthetized by Abdominal Injection with chloral hydrate （350mg/kg）, Permanent bilateral common carotid artsery ligated. 7、21 days after modeling, removed the brain tissue with HE staining. pathological changes of hippocampal neurons in rat were observed. Results：7 days and 21 days, in control group , were observed hippocampal neurons arranged rules, no significant neuronal degeneration, liquefaction necrosis and glial cell proliferation in microscope. 7 days ,in model group, were observed hippocampal neurons irregular, a small amount of neurons in the nucleus pyknosis, dissolution or disappearance,point-like liquefaction necrosis,vacuole formation, reduce the number of neurons, glial cells, a small amount of proliferation in microscope. 21 day, could be seen hippocampal neurons sparse, nucleus pyknosis, dissolution or disappearance ,dotted liquefaction necrosis, more vacuoles formed,fewer neurons, a small amount of glial cells,proliferation in microscope. Conclusion：The bilateral common carotid artery ligation in rats cauld simulated the pathogenesis of human vascular dementia （VD）, andprovide evidence hippocampal pyramidal cells, degeneration, death, loss of VD patients in morphology.