养精种玉汤乙酸乙酯部位的HPLC指纹图谱研究

Study on the HPLC Fingerprint of Ethyl Acetate Extract of Yangjing Zhongyu Decoction

目的:建立养精种玉汤乙酸乙酯提取部位的指纹图谱。方法:采用高效液相色谱法,色谱柱为Gemini C1(8250mm×4.6mm,5μm),流动相为甲醇-0.1%醋酸水溶液(梯度洗脱),检测波长为240nm,流速为1.0mL·min-1,柱温为25℃。结果:养精种玉汤的指纹图谱主要有21个特征峰,其中归属白芍的特征峰有5个,山茱萸6个,当归1个,熟地黄2个。结论:建立的指纹图谱适用于养精种玉汤及其制剂的质量控制。

OBJECTIVE： To set up the HPLC fingerprint of the ethyl acetate extract of Yangjing zhongyu decoction. METHODS： HPLC was adopted in the fingerprint analysis, and the chromatographic conditions were as follows： Gemini C18（250×4.6 mm, 5 μm） column; mobile phase consisted of methanol-0.1% acetic acid solution （gradient elution）; the detection wavelength at 240 nm; the column temperature of 25 ℃; the flow rate of 1.0 mL·min-1. RESULTS： There were mainly 21 characteristic peaks in the fingerprints. Among these peaks, 5 peaks belonged to Paeonia lactiflora, 6 peaks belonged to Cornus officinalis, 1 speak belonged to Angelica sinensis, and 2 peaks belonged to Rehmannia glutinose Praeparata. CONCLUSION： The fingerprint can be used to control the quality of Yangjing zhongyu decoction and its preparations.