广西肾综合征出血热宿主动物及其病毒感染状况

Host animals of hemorrhagic fever with renal syndrome and Hantavirus infections among host animals in Guangxi

目的调查广西壮族自治区(广西区)肾综合征出血热(HFRS)宿主动物及其自然感染汉坦病毒状况。方法用夹夜法在室内及室外捕鼠,捕获鼠经分类鉴定后无菌取血清及肺组织,用ELISA法检测血清汉坦病毒抗体及肺组织抗原,对汉坦病毒抗原阳性的肺组织标本用RT-PCR检测汉坦病毒核酸及分型。结果 2006-2008年共捕获宿主动物17种1984只,以小型啮齿动物为主,宿主动物感染汉坦病毒总抗体阳性率为14.51%;褐家鼠肺组织汉坦病毒抗原阳性标本6份,经核酸检测和测序证实为汉城型病毒。结论广西区广泛存在以褐家鼠为主要宿主的HFRS家鼠型疫源地,并存在汉城型汉坦病毒的流行。

Objective To identify the host animals of hemorrhagic fever with renal syndrome （HFRS） and their natural infections with hantavirus in Guangxi. Methods Night traps were used to capture rodents indoor and outdoor. After the rats were taxonomically identified, their sera samples and lung tissues were aseptically collected and tested for serum Hantavirus antibody and antigen in lung tissues traps using ELISA. Antigen-positive tissue samples were subject to RT-PCR to identify the viral nuclear acids and typing. Results From 2006 to 2008, 1984 host animals of 17 species were captured, dominated by small rodents. The overall positive rate of Hantavirus antibody among the host animals was 14.51%. Six positive lung tissues were detected from Rattus novegicus, which was confirmed as Seoulvirus （SEOV） through RT-PCR and sequencing. Conclusion The natural foci of HFRS were extensively present in Guangxi with R. novegicus being the major host animal. SEOV was prevalent in the region.