儿童噬血细胞性淋巴组织细胞增生症外周血淋巴细胞亚群分析

Periphery Leukomonocyte Subgroup In children with hemophagocytic lymphohistiocytosis

【目的】研究儿童噬血细胞性淋巴组织细胞增生症(hemophagocytic lymphohistiocytosis,HLH)外周血淋巴细胞活性的改变。【方法】选择2005年1月—2009年12月在本院住院的49例确诊为HLH且为急性期的患儿为病例组,30例健康儿童为对照组,分别抽取外周血,肝素抗凝,采用流式细胞术方法分别检测两组外周血淋巴细胞CD3+、CD4+、CD8+、CD(16+56)+的表达率。【结果】病例组CD(16+56)+和CD8+细胞比例显著低于对照组(P<0.01),CD3+细胞比例高于对照组(P<0.05),CD4+细胞比例与对照组比较无显著统计学意义(P>0.05)。【结论】NK细胞[CD(16+56)+]和CTL细胞(CD8+)活性的降低对诊断HLH可能具有重要临床意义,T淋巴细胞(CD3+)活性增强对诊断HLH也有一定价值。

【Objective】 To study the activity changes of Periphery Leukomonocyte in children with hemophagocytic lymphohistiocytosis（HLH）.【Methods】 The expression of CD3＋、CD4＋、CD8＋、CD（16＋56）＋on peripheral blood lym-phocytes of 49 patients with HLH（patients group） and 30 healthy children（control group） were analyzed by flow cytome-try.【Results】 Compared with the control group,the expression of CD（16 ＋ 56）、CD8 were markedly decreased（P 0.01）.The expression of CD3 was increased（P0.05）.The ratio between CD4＋and CD8＋was decreased（P 0.05）.There was no significant difference on the expression of CD4 between this two groups（P 0.05）.【Conclusions】 The decreasing activity of NK cell[（CD16＋56）＋] and CTL（CD8＋） may play an important role in the diagnosis of HLH.The increasing activity of Total T lymphocytes（CD3＋） may be valuable on the diagnosis of HLH as well.