外置式植物纤维床反应器与膜分离联用的丙酸发酵工艺研究

Propionic Acid Production in an External Plant Fibrous-Bed Bioreactor Combined with Hollow Fiber Membrane

开发了一种外置式植物纤维床反应器与膜分离联用的丙酸发酵装置,将外置式植物纤维床反应器与中空纤维膜组件相连接,即可实现丙酸的多批次稳定高效生产。实验结果表明,以Propionibacterium freudenreichii CCTCC M207015为生产菌株,葡萄糖做碳源,利用该装置发酵214h(5批次),批次平均丙酸浓度达50.77g·L-1,生产效率达1.19g·L-1·h-1,菌体干重最高达33.85g·L-1,丙酸对葡萄糖转化率和丙酸占总有机酸比例分别为50.77%、79.45%(w/w),均远优于游离细胞与膜分离联用的丙酸发酵装置。

Using glucose as carbon source and Propionibacterium freudenreichii CCTCC M207015 as the fermentation strain,a hollow fiber membrane combined with a novel external plant fibrous-bed bioreactor（PFB） was constructed and applied for the propionic acid production.The results show that the equipment could be efficient and stable for the repeated-batch propionic acid fermentation.After five batches of fermentation（214 h）,the average batch propionic acid concentration is 50.77 g·L-1 with a productivity of 1.19 g·L-1·h-1,and the maximal dry cell weight obtained is 33.85 g·L-1.Glucose conversion efficiency and the proportion of propionic acid to total organic acids are approximately 50.77% and 79.45%（w/w）,respectively.All of the data are better than that of the propionic acid production using the free cell propionic acid fermentation combined with hollow fiber membrane.