转化生长因子β_1及β_3在前列腺癌中的表达及其意义

Expression of TGF β 1 and β 3 in prostate cancer

目的研究前列腺癌（ＰＣａ）组织中转化生长因子（ＴＧＦ）β１与β３的表达及与ＰＣａ的病理分级、临床分期和远处转移的关系。方法采用免疫组织化学（ＩＨＣ）方法对４６例ＰＣａ标本进行染色。结果３９例表现广泛的肿瘤细胞外基质（ＥＣＭ）和胞浆ＴＧＦβ１染色，余７例只ＥＣＭＴＧＦβ１染色。ＴＧＦβ１ＥＣＭ／胞浆染色指数与ＰＣａＧｌｅａｓｏｎ评分、临床分期及有远处转移的ＰＣａ（Ｄ２期）呈正相关（ｒ分别为０．４２／０．３１，０．８１／０．６４，０．６８／０．４４，Ｐ均＜０．０５）。４６例ＰＣａ标本中，４５例有明显的肿瘤细胞胞浆ＴＧＦβ３染色，２５例示较弱的ＥＣＭＴＧＦβ３染色；ＴＧＦβ３染色指数与ＰＣａ临床分期、Ｇｌｅａｓｏｎ评分及远处转移无显著性相关（Ｐ均＞０．０５）。结论ＴＧＦβ１在ＰＣａ中有高表达，可能通过自分泌和旁分泌机制对ＰＣａ生长起促进而非抑制的作用，并可作为监测ＰＣａ病程进展的瘤标。ＴＧＦβ３在ＰＣａ中虽有表达。

Objective The expression of TGF β 1 and β 3 in the tissue of prostate cancer (PCa) and its correlation with the tumor grading, staging and metastasis were studied. Methods Samples of 46 ca ses with PCa were examined using immunohistochemical staining with TGF β 1 and β 3 antisera. Results Cytoplasm and extracelluar matrix (ECM) overexpression of TGF β 1 has been noted in 39 of the 46 cases with PCa while only ECM expression was noted in the other 7 samples. Statistically, there were positive correlation of the expression in ECM and cytoplasm with Gleason score, staging and metastasis ( r =0.42/0.31, 0.81/0.64, 0.68 /0.44, respectively, P <0.05 ). As for TGF β 3,45 oot of 46 samples had cytoplasm staining and 25 had weak ECM expression.There was no statistical correlation between the expression of TGF β 3 and Gleason score, staging and metastasis. Conclusions TGF β 1 may has promotive effect on the progression of cancer by autocrine with or without paracrine, and the effect of TGF β 3 in PCa is yet to be explored.