聚合酶链反应在器官移植受者人类疱疹病毒6型感染检测中的应用

Application of polymerase chain reaction in the detection of HHV6 infection in organ transplant recipients

目的探讨器官移植受者人类疱疹病毒６型（ＨＨＶ６）感染的诊断方法。方法应用聚合酶链反应（ＰＣＲ）技术，检测３１例器官移植受者术前１周、术后１６天及１０例移植术后发热时的外周血单个核细胞（ＰＢＭＣｓ）中的ＨＨＶ６ＤＮＡ。结果术前７例和术后１９例的ＰＢＭＣｓ中检测出ＨＨＶ６ＤＮＡ，１０例发热患者中８例ＨＨＶ６ＤＮＡ阳性。对１０例发热患者用阿昔洛韦治疗，８例症状得到控制，２例死亡。结论应用ＰＣＲ技术检测ＨＨＶ６感染具有简便、快速、准确、特异性高等优点。

Objective To explore the diagnosis of human herpesvirus 6 (HHV6) infection in organ transplant recipients. Methods HHV6 DNA in peripheral blood mononuclear cells (PBMCs) in 31 organ transplant recipients was detected 1 week before transplantation and 16 days after transplantation by using polymerase chain reaction (PCR). Results HHV6 DNA in PBMCs was detectable in 19 cases after operation and 7 before operation among the 31 cases respectively, and 8 out of 10 recipients with fever after operation were positive for HHV6 DNA. The 10 recipients with fever were treated by antivirus chemotherapy (acyclovir), 8 out of 10 were effective. Conclusion PCR amplification is a suitable method to diagnose and monitor HHV6 infection. The method is simple, rapid, sensitive (sensitivity 97%), and specific (specificity 98%).