利用免疫组化方法确定堆型艾美耳球虫的感染途径及部位

Definite the attack path and position of E. acervulina by immunoperoxidase staining

为了能够确定堆型艾美耳球虫(E.acervulina)在感染宿主时的途径及其进攻部位,将兔抗E.acervu-lina 3-1E基因多克隆抗体作为一抗,利用免疫组织化学方法,对鸡E.acervulina的子孢子及裂殖子进行染色。结果表明,虫体从小肠绒毛上皮细胞中感染,经绒毛内毛细血管进入肠腺,并由肠腺基底部向肠腺开口处移行,进入肠绒毛基底部的上皮细胞,逐渐扩散,最后布满整个肠绒毛。

In order to definite the attack path and position of E.acervulina.The rabbit anti-E.acervulina 3-1E polyclonal antibodies were used as the first antibody,dyeing sporozoiteby and merozoite by immunohistochemistry.The results supported that polypide invaded the host by the epithelial cells of intestinal villus,and then through the blood capillary,into the base of the intestinal gland,then to the debouch,to the base of the epithelial cell of intestinal villus,and covered all of epithelial cells of intestinal villus.