摘要
采用高效液相色谱法测定肺筋草中薯蓣皂苷的含量,建立肺筋草中薯蓣皂苷含量测定的方法。采用Shimadzu VP-ODS柱(4.6 mm×250 mm,5μm)色谱柱,V(甲醇)∶V(水)=31∶69为流动相,流速为0.5 mL/min,柱温为45℃;进样量为10μL,检测波长206 nm。研究表明:薯蓣皂苷在10.6μg/mL～106μg/mL(r=0.998 8)范围内线性关系良好,平均回收率为97.02%。此方法简便、准确、重现性好,适用于薯蓣皂苷的含量测定。
To established a method for the determination of dioscin in Aletris spicata（Thunb.） Franch by High Performance Liquid Chromatography（HPLC）.The separation was performed in a Shimadzu VP-ODS column（4.6 mm×250 mm,5 μm） with methanol water（31：69） as mobile phase.The flow rate was 0.5 mL/min.The column temperature was 45 degrees,and the detecting wavelength was at 206 nm.It was found that the linear range for dioscin was 10.6 μg/mL-106 μg/mL（r=0.998 8）,The mean recovery of dioscin was 97.02 %.This method is simple,accurate and reproducible for determination of dioscin in Aletris spicata（Thunb.） Franch..
出处
《食品研究与开发》
CAS
北大核心
2010年第12期163-165,共3页
Food Research and Development
基金
贵州省科学技术基金项目[(黔科合J字[)2008]2146号]
