产辅酶Q_(10)白地霉菌培养基的优化研究

Optimum Culture Medium of Coenzyme Q_(10) by Geotrichum candidum

以白地霉菌(Geotrichum candidum)为出发菌株,通过培养基的优化来提高辅酶Q10产量。研究碳源、混合碳源配比、氮源、混合氮源配比、无机盐、促进剂等因素对菌体干重及辅酶Q10产量的影响。确定菌种最佳培养时间为48 h,最佳培养基组成为葡萄糖32 g/L、果糖8 g/L、酵母浸粉4.5 g/L、蛋白胨1.5 g/L、Fe2+浓度23 mg/L5、0 mL发酵培养基中胡萝卜汁添加量37 mL,辅酶Q10产量达59.62 mg/L,比培养基优化前提高了25%。

Selection of Geotrichum candidum as the starting strain,through the optimization of its medium to improve the production of Coenzyme Q10,udy different carbon,the proportion of mixed carbon sources,nitrogen sources,the proportion of mixed nitrogen sources,salt,Accelerant and other factors on dry cell weight and yield of Coenzyme Q10,at last,the best time for cultivating bacteria is 48 h,the optimum medium composition for the 32 g/L glucose,fructose 8 g/L,yeast powder Baptist 4.5 g/L,Peptone1.5 g/L,Fe^2＋ concentration 23 mg/L,amount of carrot juice 37 mL in 50 mL broth,the production of Coenzyme Q10 come to 59.62 mg/L,medium before optimization than 25 %.