不同来源胶原肽的抗氧化活性及吸湿保湿性能

Antioxidant Activity,Moisture-absorbing and Moisture-retention Properties of Collagen Peptides from Different Sources

目的:对不同来源(猪皮、牛皮、驴皮、鳕鱼皮、牛骨)胶原肽的抗氧化活性和吸湿保湿性能进行比较研究。方法:采用清除DPPH自由基和ABTS+·及还原力测定进行胶原肽抗氧化活性的评价,同时对胶原肽的吸湿性和保湿性进行比较分析。结果:驴皮胶原肽具有较强的清除DPPH自由基的能力,IC50值为0.63mg/mL。鳕鱼皮胶原肽具有较强的清除ABTS+·的能力,IC50值为0.12mg/mL。驴皮胶原肽具有较强的还原力,在质量浓度10mg/mL时,OD700nm值为0.61。驴皮胶原肽具有较好的吸湿保湿性,在相对湿度43%的环境下,24h,吸湿率10.17%、72h,保湿率98.90%。结论:驴皮胶原肽具有很好的抗氧化性和吸湿保湿性。

Objective：To explore the antioxidant activity,moisture-absorbing and moisture-retention properties of collagen peptides from different sources.Methods：The antioxidant activities of collagen peptides from different sources were evaluated by determining their scavenging activities on 2,2-diphenyl-1-picrylhydrazyl（DPPH） and 2,2＇-azino-bis-2-ethybenz-thiazoline6-sulfonic acid（ABTS＋） free radicals and their reducing power.Results：The collagen peptide from donkey revealed the strongest scavenging activity on DPPH free radicals with IC50 of 0.63 mg/mL.The collagen peptide from Alaska pollack skin exhibited stronger scavenging activity on ABTS＋ free radicals with an IC50 of 0.12 mg/mL.Meanwhile,the collagen peptide from donkey also exhibited the strongest reducing power,which was 0.61 at the concentration of 10 mg/mL.The moisture-absorbing and moisture-retention rates of collagen peptide from donkey were 10.17% and 98.90% under the relative humidity of 43%.Conclusion：The collagen peptide from donkey can be used as a good additive in functional food and cosmetics.